3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA)TMB is a white crystal powder that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights.
T 5866 (OTTO) 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA - used as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein,[4] tetracosactide, sIgA and neutrophil extracellular traps (NETs).