Nile Red is an uncharged, hydrophobic, photostable molecule that strongly fluoresces bright red in hydrophobic environments.
N 1133 (OTTO) Nile red, ≥98.0% Cas 7385-67-3 - used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile Red has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation.